Identification of novel mammalian viruses in tree shrews ( Tupaia belangeri chinensis).

The Chinese tree shrew ( Tupaia belangeri chinensis), a member of the mammalian order Scandentia, exhibits considerable similarities with primates, including humans, in aspects of its nervous, immune, and metabolic systems. These similarities have established the tree shrew as a promising experimental model for biomedical research on cancer, infectious diseases, metabolic disorders, and mental health conditions. Herein, we used meta-transcriptomic sequencing to analyze plasma, as well as oral and anal swab samples, from 105 healthy asymptomatic tree shrews to identify the presence of potential zoonotic viruses. In total, eight mammalian viruses with complete genomes were identified, belonging to six viral families, including Flaviviridae, Hepeviridae, Parvovirinae, Picornaviridae, Sedoreoviridae, and Spinareoviridae. Notably, the presence of rotavirus was recorded in tree shrews for the first time. Three viruses - hepacivirus 1, parvovirus, and picornavirus - exhibited low genetic similarity (<70%) with previously reported viruses at the whole-genome scale, indicating novelty. Conversely, three other viruses - hepacivirus 2, hepatovirus A and hepevirus - exhibited high similarity (>94%) to known viral strains. Phylogenetic analyses also revealed that the rotavirus and mammalian orthoreovirus identified in this study may be novel reassortants. These findings provide insights into the diverse viral spectrum present in captive Chinese tree shrews, highlighting the necessity for further research into their potential for cross-species transmission.

Ascyrones A-E, type B bicyclic ployprenylated acylphloroglucinol derivatives from Hypericum ascyron.

Five new polycyclic polyprenylated acylphloroglucinols (1-5), ascyrones A-E, and four known compounds (6-9) were isolated from the aerial parts of Hypericum ascyron. All of the isolates containing a bicyclo[3.3.1]nonane-2,4,9-trione core and a benzoyl group, belonged to type B bicyclic polyprenylated acylphloroglucinols (BPAPs). Their structures and absolute configurations were established based on spectroscopic analyses and calculated electronic circular dichroism (ECD) data. The anti-inflammatory, neuroprotective and cytotoxicity activities of compounds 1-4 and 6-9 were evaluated. Compound 6 exhibited obvious anti-inflammatory activity in lipopolysaccharide (LPS)-induced RAW264.7 cells. Compounds 1 and 9 exhibited slight cytotoxicity against Hep3B cells. Meanwhile, compound 1 showed mild neuroprotective activity against corticosterone (CORT)-induced PC12 cell damage at 10 µmol·L-1.

Histone deacetylase­2: A potential regulator and therapeutic target in liver disease (Review).

Histone acetyltransferases are responsible for histone acetylation, while histone deacetylases (HDACs) counteract histone acetylation. An unbalanced dynamic between histone acetylation and deacetylation may lead to aberrant chromatin landscape and chromosomal function. HDAC2, a member of class I HDAC family, serves a crucial role in the modulation of cell signaling, immune response and gene expression. HDAC2 has emerged as a promising therapeutic target for liver disease by regulating gene transcription, chromatin remodeling, signal transduction and nuclear reprogramming, thus receiving attention from researchers and clinicians. The present review introduces biological information of HDAC2 and its physiological and biochemical functions. Secondly, the functional roles of HDAC2 in liver disease are discussed in terms of hepatocyte apoptosis and proliferation, liver regeneration, hepatocellular carcinoma, liver fibrosis and non­alcoholic steatohepatitis. Moreover, abnormal expression of HDAC2 may be involved in the pathogenesis of liver disease, and its expression levels and pharmacological activity may represent potential biomarkers of liver disease. Finally, research on selective HDAC2 inhibitors and non­coding RNAs relevant to HDAC2 expression in liver disease is also reviewed. The aim of the present review was to improve understanding of the multifunctional role and potential regulatory mechanism of HDAC2 in liver disease.

Transcriptome Analysis of Needle and Root of Pinus Massoniana in Response to Continuous Drought Stress.

Pinus massoniana Lamb. is an important coniferous tree species in ecological environment construction and sustainable forestry development. The function of gene gradual change and coexpression modules of needle and root parts of P. massoniana under continuous drought stress is unclear. The physiological and transcriptional expression profiles of P. massoniana seedlings from 1a half-sibling progeny during drought stress were measured and analyzed. As a result, under continuous drought conditions, needle peroxidase (POD) activity and proline content continued to increase. The malondialdehyde (MDA) content in roots continuously increased, and the root activity continuously decreased. The needles of P. massoniana seedlings may respond to drought mainly through regulating abscisic acid (ABA) and jasmonic acid (JA) hormone-related pathways. Roots may provide plant growth through fatty acid ß-oxidative decomposition, and peroxisomes may contribute to the production of ROS, resulting in the upregulation of the antioxidant defense system. P. massoniana roots and needles may implement the same antioxidant mechanism through the glutathione metabolic pathway. This study provides basic data for identifying the drought response mechanisms of the needles and roots of P. massoniana.

[Theoretical snow period and assessment of skiing climate suitability: With Changbai Mountain Ski Resort as an example]. / 理论雪期和滑雪气候适宜度评价­­ä»¥é•¿ç™½å±±æ»‘雪场为例.

To analyze the climatic characteristics of snow resources and quantitatively evaluate the climatic suitability of skiing, we proposed the concept of theoretical snow period from the perspective of climate based on the synoptic principle. We set threshold values of different suitability degrees of three indices closely related to skiing, including air temperature, wind speed, and precipitation. The conversion function of each index was designed after normalization. Based on grey relational theory and Euclidean distance method, we established evaluation model of ski sports climate suitability index, with Changbai Mountain Ski Resort as an example. The results showed that snow resource in Changbai Mountain area was rich. From 1981 to 2018, the average snowfall during the theoretical snow period was 64.6 mm. Under the background of climate change, the average number of snow days and snowfall decreased slightly over the years, with the starting time of snow season being delayed and the ending time being advanced. The number of snow days in the early winter (from the starting time of snow season to the end of December) was significantly less than that in the later winter (from the next January to the end of snow season). Climate in Changbai Mountain was highly suitable for skiing during the snow period. The most suitable and relatively suitable days with respect to air temperature, wind speed and precipitation accounted for 91.9%, 91.8%, and 94.6% of the total, respectively. The cumulative number of days for ski comprehensive weather suita-bility accounted for 99.7%, indicating that most days were suitable for skiing. The concept of theoretical snow period in this study had made up for the problems caused by the lack of meteorological observation data of the first and last snow event on the study of snow resources. The climate suitabi-lity evaluation model of skiing could help make management decision for the development and operation of ski resorts and scientific support for skiing enthusiasts.

Triketone-terpene meroterpenoids from the leaves of Rhodomyrtus tomentosa.

Eight new meroterpenoids (1-8) featuring ß-triketone-conjugated terpenoids, rtomentones A-H, were isolated from the leaves of Rhodomyrtus tomentosa. Structures of the isolates were unambiguously established by a combination of NMR and ECD spectroscopy and X-ray diffraction analysis. Rtomentone C (3) was the first example of aromadendrane-based meroterpenoid containing an oxa-spiro[5.6] ring. Rtomentone D (4) was obtained as a racemic mixture confirmed by chiral HPLC analysis. The cytotoxicity against MDA-MB-231, A549, and DLD-1 cells of all isolates was evaluated.

Reproductive biology characteristic of Jatropha curcas (Euphorbiaceae)

Jatropha curcas belongs to family of Euphorbiaceae, and is an important biological tree species for diesel production. The current descriptions of the phenotypic traits for male and female flowers are not comprehensive and there have been no reports about the process of J. curcas from pollen germination on stigma to pollen tubes conducting fertilization after entering the ovary and ovule. To assess this, experiments were undertaken to study the reproductive biology characteristic of J. curcas in Guiyang Guizhou Province, China. Floral structure and pollen germination process were described in detail and the breeding system was determined. The results showed that flower of J. curcas was both unisexual and monoecious, with a flowering phase between April-November. Both female and male flowers have five petals in contorted arrangement and five calyxes in imbricated arrangement. Female flower originated from bisexual flower finally formed unisexual flowers as the stamen ceased growth in different period. The pistil had 3-5 styles, connected at base and separated into 3-5 stigmas on the top. Each stigma had 2-4 lobes. The styles were hollow. The pollen germinated on the surface of the stigma, is then transported via the vascular tissues, which was arranged in bundles, and finally channeled through the micropyle to enter the blastula. The pollen tube was shaped in a long uneven cylinder. The top end of it became swollen and formed a small round hole for the purpose of releasing sperm nuclei while the pollen tube itself was growing and extending. Estimation of out-crossing index and artificial pollination experiments indicated that J. curcas was capable of both self-pollination and cross-pollination. The germination speed of the pollen on the stigma did not differ so much between the one by self-pollination and the one by cross-pollination, and the pollen from the two different sources could both reach the ovary within one day. Both artificial ...

Jatropha curcas pertenece a la familia Euphorbiaceae, y es una importante especie arbórea para la producción de diesel. Las descripciones actuales de los rasgos fenotípicos de flores masculinas y femeninas no son exhaustivas y no hay informes sobre la germinación del polen en el estigma, que viaja a través de los tubos polínicos hasta el ovario y se de la fertilización de los óvulos de J. curcas. Para evaluar esto, se llevaron a cabo experimentos para estudiar la biología reproductiva de J. curcas en la provincia de Guizhou, Guiyang, China. La estructura floral y el proceso de germinación de polen se describen en detalle y se determinó el sistema reproductivo. Los resultados mostraron que las flores de J. curcas eran a la vez unisexuales y monoicas, la floración se dio entre abril y noviembre. Las flores femeninas y masculinas tienen cinco pétalos en disposición retorcida y cinco cálices en disposición imbricada. La flor femenina originada de flores bisexuales finalmente formo flores unisexuales, cuando el estambre ceso el crecimiento en un período diferente. El pistilo tuvo 3-5 estilos, unidos en la base y separados en 3-5 estigmas en la parte superior. Cada estigma tenía de 2-4 lóbulos. Los estilos eran huecos. El polen germinado en la superficie del estigma, se transporta a través de los tejidos vasculares, que se disponen en haces, y finalmente son canalizados a través del micrópilo para entrar a la blástula. El tubo polínico tenía forma de cilindro irregular largo. El extremo superior del mismo se hincho y formó un pequeño agujero redondo con el fin de liberar los núcleos de esperma mientras que el tubo de polen crecía y se extendía. Estimaciones del índice de cruzamiento y experimentos de polinización artificial indicaron que J. curcas era capaz tanto de auto-polinización como de polinización cruzada. La velocidad de germinación del polen en el estigma no difiere mucho entre una planta con autopolinización y otra con polinización ...

Reproductive biology characteristic of Jatropha curcas (Euphorbiaceae).

Jatropha curcas belongs to family of Euphorbiaceae, and is an important biological tree species for diesel production. The current descriptions of the phenotypic traits for male and female flowers are not comprehensive and there have been no reports about the process of J. curcas from pollen germination on stigma to pollen tubes conducting fertilization after entering the ovary and ovule. To assess this, experiments were undertaken to study the reproductive biology characteristic of J. curcas in Guiyang Guizhou Province, China. Floral structure and pollen germination process were described in detail and the breeding system was determined. The results showed that flower of J. curcas was both unisexual and monoecious, with a flowering phase between April-November. Both female and male flowers have five petals in contorted arrangement and five calyxes in imbricated arrangement. Female flower originated from bisexual flower finally formed unisexual flowers as the stamen ceased growth in different period. The pistil had 3-5 styles, connected at base and separated into 3-5 stigmas on the top. Each stigma had 2-4 lobes. The styles were hollow. The pollen germinated on the surface of the stigma, is then transported via the vascular tissues, which was arranged in bundles, and finally channeled through the micropyle to enter the blastula. The pollen tube was shaped in a long uneven cylinder. The top end of it became swollen and formed a small round hole for the purpose of releasing sperm nuclei while the pollen tube itself was growing and extending. Estimation of out-crossing index and artificial pollination experiments indicated that J. curcas was capable of both self-pollination and cross-pollination. The germination speed of the pollen on the stigma did not differ so much between the one by self-pollination and the one by cross-pollination, and the pollen from the two different sources could both reach the ovary within one day. Both artificial pollination test and out-crossing index have indicated that: J. curcas has both self-pollination and cross-pollination systems.

[Interactive effects of P deficiency and Al toxicity on soybean growth: a pot experiment with acid soil].

Taking seven soybean genotypes differing in P efficiency as test materials, a pot experiment with acid soil was conducted to study the interactive effects of P deficiency and Al toxicity on the growth of the genotypes under liming and P fertilization. The results showed that P deficiency and Al toxicity were the limiting factors for the soybean growth on acid soil, and the impact of Al toxicity was more severe. There existed interactive effects between P deficiency and Al toxicity. Comparing with liming or P fertilization alone, their combination could greatly promote soybean growth. The impact of P deficiency and Al toxicity on the P uptake by soybean was far greater than that on N and K uptake. A reasonable planting system for soybean would be helpful for the amelioration of acid soil. The combined application of 1.103 g Ca(OH)2 x kg(-1) soil and 2.018 g KH2PO4 x kg(-1) soil) could increase the acid soil pH by 38.4% and the available P content by 3223.8%, and decreased the soil exchangeable Al content by 96.3%. Planting P-efficient soybean genotypes rather than P in-efficient soybean genotypes had better effect in acid soil amelioration.

[Method for drainage of lymph fluid and determining the change of active materials in lymph fluid after rat ischemia-reperfusion injury].

OBJECTIVE: To set up a method for the drainage of lymph fluid and explore the change of active materials in lymph fluid and serum after rat ischemia-reperfusion injury. METHODS: The method of the drainage of lymph fluid was well established. Sixteen healthy male rats of SPF grade were selected and randomly divided into 2 groups: intestinal ischemia-reperfusion + drainage group (I/R + drainage group) and drainage group. All the rats were subjected to superior mesenteric artery occlusion for 60 minutes, followed by 120 minutes of reperfusion. We compared the change of high mobility group box-1 (HMGB1) protein, endotoxin tumor necrosis factor alpha (TNF-alpha), interleukin (IL) -1 beta, IL-6, and soluble vascular cell adhesion molecular-1 (sICAM-1) by draining lymph fluid and collecting serum in 2 groups. RESULTS: The drainage of lymph fluid was successfully performed. The HMGB1, endotoxin, and cytokines in serum and lymph fluid were significantly higher in ischemia-reperfusion group than in drainage group (P < 0. 05). CONCLUSIONS: The method for drainage of lymph fluid is simple and feasible. Endotoxin, HMGB1, and some cytokines in serum and lymph fluid may mediate the ischemia-reperfusion injury.

Similar ability of FbaA with M protein to elicit protective immunity against group A streptococcus challenge in mice.

Group A streptococcus (GAS), an important human pathogen, can cause various kinds of infections including superficial infections and potentially lethal infections, and the search for an effective vaccine to prevent GAS infections has been ongoing for many years. This paper compares the immunogenicity and immunoprotection of FbaA (an Fn-binding protein expressed on the surface of GAS) with that of M protein, the best immunogen of GAS. Assay for immune response showed that FbaA, similar to M protein, could induce protein-specific high IgG titer in BALB/c mice. Furthermore, following GAS challenge, the mice immunized with FbaA showed the same protective rate as those with M protein. These results indicate that FbaA is similar in ability to M protein in inducing protective immunity against GAS challenge in mice.

[Health care status of female workers exposed to occupational hazards in Haidian district of Beijing].

OBJECTIVE: To investigate the health care status of female workers exposed to occupational hazards in Haidian district of Beijing and improve the labor protection of female workers. METHODS: A questionnaire provided by National Center for Women and Children's Health of Chinese CDC was used in the survey conducted to collect information about health care status of female workers in 141 factories with occupational hazards including chemical poisons and physical factors (noise, libration, microwave, high frequency and low temperature). RESULTS: 141 factories were investigated, including 53 state-owned enterprises, 21 collective enterprises, 46 joint-stock enterprises, and 21 non-public enterprises. 12 251 female workers were surveyed, 10.19% (1249/12 251) of whom were exposed to occupational hazards. Of 141 factories studied, 16.31% (23/141) had no labor protection management organization.27.66% (39/141) did not provide pre-employment physical examination service to female workers.48.94% (69/141) didn't establish labor protection system for female workers in menstrual period. While, 21.28% (30/141) of the studied institutes deducted some salaries in the pregnancy, and 32.62% (46/141) deducted their wages during the puerperal period. 2.13% (3/141) arranged female workers in the posts which are forbidden by law (continuous heavy work load operation).9.93% (14/141) arranged pregnant female workers on the post forbidden by law.31.91% (45/141) and 33.33% (47/141) would deduct the time of prenatal medical examination and lactation from their working hours, respectively.39.01% (55/141) didn't afford the cost of fertility. 68.09% (96/141) had annual gynecological examination.45 factories were collected occupational examination reports, accounted for 31.91% (45/141). No female workers were found suffering from occupational disease. Of the 1865 occupational hazard factor monitoring points in 34 factories, there were 155 monitoring points, which were all noise monitoring points, did not meet the standard. CONCLUSION: The current health-care status of female workers is not optimistic. It is necessary to consistently improve health care legislations, establish coordinated management mechanism and strengthen the publicity of policy to protect female workers.

[Regulation effect of soil P availability on mycorrhizal infection in relation to root architecture and P efficiency of Glycine max].

A pot experiment with thirty soybean (Glycine max) genotypes differing in root architecture was conducted to investigate the effects of soil phosphorus (P) availability on the colonization of arbuscular mycorrhizae (AM) fungus (Glomus mosseae) as well as the relationships of the colonization with the root architecture and P efficiency of soybean. The results indicated that soil P availability had significant effects on the colonization of G. mosseae. Under low P condition, the AM had a higher infection rate and contributed more to the P uptake by soybean; while under high P condition, it was in adverse. There was a significant interaction between soybean genotypes and soil P availability on the colonization of G. mosseae. Under low P condition, the soybean genotypes with intermediate and deep root architectures had the highest infection rate of AM, but on the contrary, no significant correlation was observed between root architecture and AM colonization under high-P condition. In general, P-efficient soybean genotypes had better root architecture or higher AM colonization, indicating that a complementary relationship between root architecture and AM colonization was existed on their contribution to soybean P efficiency.

[Human papillomavirus genotype distribution in invasive squamous cell carcinoma of the uterine cervix in Mongolian women in inner Mongolia autonomy region].

OBJECTIVE: To investigate the prevalence of human papillomavirus (HPV) and the HPV genotype distribution in invasive squamous cell carcinoma of the uterine cervix in the Mongolian women in Inner Mongolia autonomy region. METHODS: The prevalence data of HPV in our department were retrospectively reviewed. INNO-LiPA genotyping technique was used to detect HPV genotypes in the reserved carcinoma tissue specimens. RESULTS: Totally 63 tissue specimens were collected and detected. The prevalence of HPV was 93.7%. The positive rates of HPV among different clinical staging and different pathological grading were not significantly different (P >0.05). The prevalence of HPV16 was not significantly different among different age groups (P>0.05). HPV16 (69.8%), HPV18 (4.8%), HPV31 (4.8%), HPV39 (4.8%), and HPV52 (3.2%) were the 5 dominating HPV genotypes in all cases. CONCLUSIONS: HPV infection is closely correlated with invasive squamous cell carcinoma of the uterine cervix in Mongolia women. HPV16 is the most important genotype in invasive squamous cell carcinoma of the uterine cervix, followed by HPV18, 31, and 39. HPV infection dose not affect the progression and differentiation of invasive squamous cell carcinoma of the uterine cervix.

[Analysis of maltose clearance in plasma and urine of healthy volunteers with high-performance liquid chromatography].

OBJECTIVE: To analyze the maltose clearance in plasma and urine of healthy volunteers with high-performance liquid chromatography. METHODS: Maltose solution was infused to 12 healthy volunteers during a 4-hour period at an infusion rate of 0.2, 0.3, and 0.5 g/(kg x h), Plasma and urine specimens were collected at different time points before and after infusion, and then analyzed with high-performance liquid chromatography. RESULTS: The coefficients of variation of the precision and accuracy of the analysis method ranged 3.68%-4.58% and 0.44%-4.83% for plasma, respectively, and 2.91%-7.62% and 0.95%-8.27% for urine, respectively. The plasma maltose concentration increased in a dose-dependent manner (r > 0.99). The plasma maltose concentrations returned to the baseline levels 12 hours later. Two hours after injection, the urinary excretion of maltose increased, reached the peak value within 2-4 hours, began to decrease 6 hours later, and became zero 24 hours later. CONCLUSIONS: An infusion rate of 0.2-0.5 g/(kg x h) of maltose will not remarkably increase the blood glucose level in healthy people. The routine infusion rate should below 0.3 g/(kg x h), unless an emergency exists.

[Study on transdifferentiation of renal tubular cells in rat chronic renal interstitial fibrosis induced by Radix Aristolochiae Fangchi Extract].

OBJECTIVE: To investigate the relationship between renal tubular cells transdifferentiation and chronic renal interstitial fibrosis induced by Fangchi Extract in rat. METHOD: The chronic renal interstitial fibrosis rat model was made by giving Radix Aristolochiae Fangchi extract (RAFE) and aristolichic acid (AA) respectively to rats through infusing stomach about 22 weeks discontinuously. Through immunnal histochemistry methods, investigating the expression of symbol proteins: Cytokine( CK) , alpha-Smooth muscle actin ( alpha-SMA) and Vimentin, and also the important fibrosis inducing factor-Transforming growth factor-beta (TGF-beta1 )on renal tubular cells. RESULT: In RAFE and AA Groups, the expression of CK on renal tubular cells is declined comparing with the Control Group, and the enhanced expression of alpha-SMA and Vimentin can be observed on tubular cells. The expression of TGF-beta1 on renal tubular cells stronglhy increased, too. CONCLUSION: Part of the renal tubular cells was transdifferentiated into myofibroblasts. Renal tubular cells may participate the occurance of chronic renal interstitial fibrosis, TGF-beta1 may accelerate the transdifferentiation of tubular cells.

[Experimental study of chronic renal tubular-interstitial injury induced by Radix Aristolochiae Fangchi Extract in rats].

OBJECTIVE: Following the former report, we continue to observe the chronic renal tubular-interstitial injury induced by Radix Aristolochiae Fangchi Extract(RAFE) in rats in order to understand whether RAFE in different doses causes the renal tubular-interstitial injury or not. METHOD: RAFE at the dose of 25.0 mg x kg(-1) x d(-1), 120.0 mg kg(-1) x d(-1) and 200.0 mg x kg(-1) x d(-1) and aristolochic acid (AA, 10.0 mg x kg(-1) d(-1)) was interruptedly administrated by gastric tube for 22 w and 4 w durg withdrawal. Blood, urine and kidney were taken out respectively in 17 w, 22 w and 26 w to measure the indexes of renal function. The morphology of kidney was observed, and Masson staining of kidney were made respectively to compare RAFE groups with AA group. RESULT: Pathological changes of renal tissue forms were as follows: All RAFE groups and AA group could develop the pathological process of renal tubular injury-chronic renal interstitial fibrosis. The pathologic changes of RAFE were similar with AA. CONCLUSION: RAFE at all doses administrated interruptedly by gastric tube above 13 w caused chronic renal tubulo-interstitium fibrosis. The renal injury in functions and tissue forms in rats were similar with AA closely. The results showed that AA was the main toxic composition of RAFE.

Role of perioperative parenteral nutrition in severely malnourished patients with Crohn's disease.

AIM: To evaluate the effect of perioperative parenteral nutrition on serum immunoglobulin, weight change, and post-operative outcome in severely malnourished patients with Crohn's disease. METHODS: Thirty-two severely malnourished patients with Crohn's disease who had undergone surgery in our hospital were reviewed. Sixteen patients who received perioperative parenteral nutrition were enrolled in the study group, and the other 16 patients who did not receive parenteral nutrition were enrolled in the control group. Serum immunoglobulin, body mass index (BMI), liver function, weight change, and postoperative complications were evaluated. RESULTS: Serum IgM levels elevated 1 wk before surgery in both groups, and decreased to normal value (from 139+/-41 to 105+/-29 mg/dL, P = 0.04) 4 wk after operation in the study group, while no significant changes was noted in the control group (from 133+/-16 to 129+/-13 mg/dL, P = 0.34). There were no significant changes in concentrations of IgG and IgA. The BMI of the study group increased from 13.9+/-0.6 to 15.3+/-0.7 kg/m(2) (P = 0.02) with no significant change in the control group (14.1+/-0.7 and 14.5+/-0.5, respectively, P = 0.81). The percentage of resuming work was higher in the study group than in the control group. CONCLUSION: Perioperative parenteral nutrition possibly ameliorates the humoral immunity, reverses malnutrition, and facilitates rehabilitation.

[Therapeutic effect of Qingkailing and methylprednisolone injection alone or combined on the acute lung injury induced by oleic acid in rabbits].

OBJECTIVE: To investigate the effect of Qingkailing and Methylprednisolone (MP) injection alone or combined on the acute lung injury (ALI) induced by oleic acid in rabbits. METHOD: The rabbits were randomly divided into 11 groups: oleic acid group; control group; treatment groups including low, middle and high dosage groups of Qingkailing and MP alone and combined, respectively. ALI model was established by i.v. oleic acid (0.05 mL x kg(-1)) in these groups, and then i.v. above drugs respectively, while in control group, the same volume of normal saline was given. The respiratory amplitude and rate were observed, and blood samples were taken from cervical artery for blood-gas analysis before and at 30, 60, 120 min after oleic acid or normal saline administration. At the end of experiment, the concentration of LDH, CAT and MDA in the lung tissue were measured and pathologic changes of lung tissue were observed microscopically. RESULT: Compared with oleic acid group, the respiratory amplitude markedly enhanced (P < 0.05) and respiratory rate lowered (P < 0.05) in the low, middle and high dose groups of Qingkailing and MP injection. On the 30 min of treatment, PaO2 increased significantly (P < 0.05) in the low and middle dose groups of combined Qingkailing and MP injection; PaCO2 decreased markedly (P < 0.05) on the 120 min of treatment in each treatment group. The level of LDH significantly increased (P < 0.05), CAT and MDA decreased (P < 0.05) in the middle and high groups of Qingkailing and MP injection. The low and middle dose groups of combined Qingkailing and MP injection can alleviate the pathological changes induced by oleic acid. CONCLUSION: The curative effect of the low dose group of combined Qingkailing and MP for the ALI induced by oleic acid was better than Qingkailing and MP alone, while the big dose groups of Qingkailing and MP alone better than the combination at the same dosage.